Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate
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چکیده
منابع مشابه
Kinetic colorimetric assay of lipase in serum.
We describe a kinetic colorimetric method for assaying lipase (EC 3.1.1.3) activity in serum by using a natural long-chain fatty acid 1,2-diglyceride. In the presence of colipase, deoxycholate, and calcium ions, pancreatic lipase hydrolyzes the clear substrate solution to produce a 2-monoglyceride, which in turn releases glycerol by the action of a 2-monoglyceride lipase. Glycerol is then assay...
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A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate-Cibachron Blue-amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chro. mogen, the formation of which islinearwith enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 mm or, in international units, 78 ± 24 (...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 2001
ISSN: 0004-5632,1758-1001
DOI: 10.1258/0004563011900876